Author

Sawan Hurst

Date

August 2010

Document Type

Thesis

Degree Name

M.S.

Department

Dept. of Biomedical Engineering

Institution

Oregon Health & Science University

Abstract

We have developed a new optical micro-angiography (OMAG) imaging technique that is capable of resolving 3D distribution of dynamic blood perfusion at a 10 µm resolution within icrocirculatory beds in vivo. The imaging contrast of blood perfusion is based on endogenous light scattering from moving blood cells within vessels, thus no exogenous contrast agents are necessary. The technique effectively separates the moving and static scattering elements within tissue to achieve high resolution images of blood flow, mapped into the 3-D optically sectioned tissue beds, at speeds that allow for perfusion assessment in vivo. We used OMAG to visualize the cerebral microcirculation of adult living mice through the intact cranium. These measurements would be difficult, if not impossible, with other optical imaging techniques. The purpose of this study is to validate OMAG as a method and assess its potential applications in the studies of cerebrovascular perfusion during and after induced stroke in treated mice. To do this we treated animals with tissue plasminogen activator (tPA) or vehicle and then used OMAG to obtain real time images to compare the mechanisms of reperfusion of each treatment. From these images tPA is shown to greatly effect the reperfusion. It is shown that tPA increases the reperfusion rate and blood volume into the affected hemisphere which in turn increases the animals’ behavior and survival chances.

Identifier

doi:10.6083/M4XK8CHC

School

School of Medicine

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