Date

6-1-2013

Document Type

Thesis

Degree Name

M.S.

Institution

Oregon Health & Science University

Abstract

Introduction: Microorganisms are vulnerable to invasion by mobile genetic elements such as viruses, plasmids and transposons. The recently discovered CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-associated system (CRISPR-Cas) is an adaptive immunity system found in most archaea and many bacteria that targets and inactivates invading foreign genetic elements. Cells with CRISPR-Cas are more likely to resist the invasion and uptake of foreign DNA such as viruses, plasmids and transposons. Several CRISPR-Cas systems have been identified in prokaryotes, including CRISPR1-cas and CRISPR3-cas in E. faecalis isolates recovered from human, animal, insect and environmental sources. The aims of this study were to: (1) compare the occurrence of CRISPR-cas in collections of endodontic (n=34), oral (n=21), and multidrug-resistant hospital-acquired strains of E. faecalis (n=23); (2) evaluate the distribution of antibiotic resistance and virulence traits amongst strains without C

Comments

This document has been embargoed indefinitely and consequently is not available in full text electronic format. A circulating bound text of the document is available in the stacks of the Library.

Identifier

doi:10.6083/M4765CB0

School

School of Dentistry

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