Dept. of Molecular Microbiology and Immunology
Oregon Health & Science University
Considerable progress has been made in recent years, reinvigorating the HIV NAb field. Nevertheless, important questions remain concerning Env structure that can lead to the development of an immunogen that can elicit hNAbs. In this regard, recent evidence demonstrates that sequential immunization with HIV-1 Envs that evolved in a macaque infected with pathogenic SHIV leads to broadening of NAbs in rabbits, and that these NAbs are more similar to the NAbs generated durig SHIV infection (253). The aim of the work presented here is to extend these data using human-derived envs. I hypothesize that envs derived from the viral quasispecies of HIV-infected subjects that develop broad NAbs contribute to the development of this response; moreover, these Env variants develop particular immunogenic features that drive Env-specific B cells down a particular maturation pathway as they diverge over the course of infection, leading to broad NAbs. In Chapter Two, I explore the using a rational method
School of Medicine
Pissani, Franco, "HIV vaccine design based on in vivo evolution of quasispecies envelope proteins" (2012). Scholar Archive. 887.