Dept. of Chemistry and Biochemical Sciences
Oregon Graduate Center
The white-rot basidiomycete Phanerochaete chrysosporium produces intracellular soluble and particulate Ã-glucosidases and an extracellular Ã-glucosidase. The extracellular enzyme is induced by cellulose but repressed in the presence of glucose. The molecular weight of this enzyme is 90,000. The K [subscript m] for -p-nitrophenyl-Ã-glucoside is 1.6 x 10[superscript -4] M; the K [subscript 1] for glucose, a competitive inhibitor, is 5.0 x 10[superscript -4] M. The K [subscript m] for cellobiose is 5.3 x 10[superscript -4] M. The intracellular soluble enzyme is induced by cellobiose; this induction is prevented by cycloheximide. The presence of 300 mM glucose in the medium, however, had no effect on induction. The K [subscript m] for p-nitrophenyl- Ã -glucoside is 1.1 x 10[superscript -4] M. The molecular weight of this enzyme is about 410,000. Both enzymes have an optimal temperature of 60Â°C and an E [subscript act] of 9.15 kcal/mole. The pH optima, however, were 7.0 and 5.5 for the intracellular and extracellular enzymes, respectively.
Smith, Mark H., "Phanerochaete Chrysosporium B-Glucosidases: induction, cellular localization, and physical characterization" (1980). Scholar Archive. 211.