Dept. of Biochemistry and Molecular Biology
Oregon Graduate Institute of Science & Technology
Neurospora crassa arg-2 encodes the small subunit of Arg-specific carbamoyl phosphate synthetase and is negatively regulated by arginine. This regulation is mediated by a 24-codon upstream open reading frame (uORF). The sequence of this uORF is critical for Arg-specific regulation. Six mutated templates were used to examine which residues of the uORF are important for this regulation. Mutations were created using megaprimer PCR and a luciferase gene was used as a reporter in the in vitro translation studies. Mutations of Asp 12, Asp 16, and Ser 10 eliminate Arg-specific regulation. Leaky scanning is thought to be involved, and a hypothetical ribosome stalling model that mediates Arg-specific attenuation of translation is proposed.
Carroll, Julie Marie, "Arginine-specific negative regulation of Neurospora crassa arg-2 mediated by the arg-2 uORF and Arginine" (1997). Scholar Archive. 285.