Iyad A. Sood


May 2005

Document Type


Degree Name



Dept. of Periodontology


Oregon Health & Science University


Considerable interest has been generated recently, concerning the effect of surface roughness on osteoblast attachment and proliferation. Objectives: This study characterized attachment and proliferation of rat preosteoblast cells on discs of material having differing surface textures. Methods: Discs were made of Ti-6A1-4V alloy and each disc measured 15.5mm in diameter and 1.5mm thick. Test disc samples were provided by Center Pulse Dental, Inc.® (now Zimmer Dental®) and were textured to evaluate 3 selected surface parameters: pit spacing, pit diameter, and pit depth (see Table 1 and 2). The test discs were designated as: D2 (close-small-deep); D3 (close-large-shallow); D6 (far-small-shallow); D7 (far-large-deep). D4 (blasted), D5 (HA coated), and D8 (machined) discs were used as control discs. D1 (polystyrene) consisted of empty cell culture treated wells that did not contain a disc were used as positive controls. The discs were used as substrates upon which the osteoblast cells were cultured onto 24-well culture polystyrene plates. To obtain pre-osteoblasts, calvarae from day 19 fetal Sprague Dawley rats were pooled and treated with collagenase to liberate the cells. Cells were allowed to recover for three days prior to plating. Quantification of cell viability within the test wells was measured at predetermined times of 4, 24, 48, 96, 120, 144, and 168 hours by observing the reduction of 10% Alamar Blue nuclear dye using a fluorescent colorimeter. The resulting measurements from the Alamar Blue dye analyses were then analyzed and reviewed for possible trends or differences between the different surface textures using ANOVA at 95% confidence interval (p<0.05). Results: Discs with far parameters had statistical significant greater cell numbers than discs closer together at 72 hours (p<0.05). There were no observed differences in cell numbers between the shallow and deep pits. The 4 experimental discs resulted in greater cell numbers than D5 (HA coated) at all time points except 72 hours. D3 (cl-lg-sh) showed greater cell numbers than D4 (blasted) at 96 and 144 hours. Also, D3 (cl-lg-sh) resulted in greater cell numbers than D5 (HA coated) at all times except 72 hours. D4 (blasted) showed greater cell numbers than D5 (HA coated) at all time points except at 72 and 96 hours (p<.05). Conclusions: Within the limitations of this study, it appears that the pitted surfaces generally have a greater cell attachment and proliferation than the HA coated; however, machined surface textures showed the greatest number of cells, suggesting that size of texture is not necessarily as important as type of surface.




School of Dentistry



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