Dept. of Cell and Developmental Biology
Oregon Health & Science University
Tumor-initating cells (TICs) are operationally defined as tumor cells with the ability to initiate tumor formation upon grafting into immunocompromised mice. TICs have been characterized as drug resistant and as possible sources of metastatic cells, although the latter function is poorly understood. In order to gain a better understanding of the role of these cells in metastasis, we have studied TICs from a mouse model of skin squamous cell carcinoma (SCC) initiated by activation of Kras and inactivation of Smad4 in the hair follicle bulge stem cells. These mice develop tumors that are metastatic to the lung. Lung metastasis in this model was shown to be greatly increased by xenografting (passaging) the tumors. Two TIC populations were defined within this tumor model, the Hoechst dye excluding side population (SP) and the SP[superscript -]/CD34[superscript +]/CD49f[superscript +] population. We observed that the size of the SP, but not the SP[superscript -]/CD34[superscript +]/CD49f[su
School of Medicine
White, Ruth, "MicroRNA regulation of tumor-initiating-cells in metastasis and chemoresistance" (2011). Scholar Archive. 680.